Dixie J. Goss Group
Our lab is using biophysical methods and molecular biology to study protein-nucleic acid interactions and macromolecular assembly.
We are particularly interested in mechanisms of assembly and how kinetics and equilibria influence the final composition of assembly complexes and ultimately biological function. Experiments are aimed at determining the detailed function of proteins and how they recognize nucleic acids. These processes are studied by steady-state and life-time fluorescence measurements, circular dichroism, rapid scanning stopped-flow, and site-directed mutagenesis. Detailed quantitative measurements of these biological processes lead to elucidation of molecular mechanisms and how these processes can be regulated.